Antibody data

Product number
11-5698-80
Provider
Invitrogen Antibodies
Product name
Anti-Ki-67 Monoclonal Antibody (SolA15), FITC, eBioscience™
Provider product page
Invitrogen Antibodies - 11-5698-80
Antibody type
Monoclonal
Antigen
Other
Description
Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin). The SolA15 antibody also recognizes human, non-human primate and canine Ki-67. Applications Reported: This SolA15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining, and immunocytochemistry. Applications Tested: This SolA15 antibody has been tested by immunocytochemistry on fixed and permeabilized OLN93 cells at less than or equal to 10 µg/mL. It has also been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat 00-5523) and protocol. For flow application this can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse, Rat, Canine
Host
Rat
Conjugate
Green dye
Isotype
IgG
Antibody clone number
SolA15
Vial size
25 µg
Concentration
0.5 mg/mL
Storage
4° C, store in dark, DO NOT FREEZE!